Equipment/Services

Primary use: Untargeted and semi-untargeted metabolite profiling and stable isotope labeling

Capabilities

• High-resolution/accurate mass data acquisition
• Up to four chromatographic separations per sample to maximize metabolite coverage
• MS2 fragmentation for compound identification and library matching
• MSn fragmentation for structural elucidation

Specifications

• Up to 500k resolution (FWHM)
• < 2ppm mass accuracy
• Electrospray ionization
• Dual Vanquish UHPLC
• Data dependent acquisition
• CID and HCD fragmentation

Primary use: Targeted polar metabolite profiling and custom targeted method development

Capabilities

• Ion-paired negative mode profiling of ~250 polar metabolites, including amino acids, nucleotides, carboxylates and sugar phosphates
• dMRM mode limits transition monitoring only to retention windows of interest to maximize compounds detected per run
• Highly reproducible ion-paired chromatography
• High sensitivity and specificity of targeted compounds
• Second UHPLC system for targeted method development of compounds not amenable to ion-pairing.

Specifications

• Sub-femtogram detection limits (compound dependent)
• Electrospray ionization

Primary use: Targeted metabolite profiling of central carbon metabolism and custom targeted method development

Capabilities

• Profiling, quantitation, and stable isotope labeling of most amino acids and TCA cycle intermediates
• In-source compound fragmentation for positive ID

Specifications

• Sub-femtogram detection limits (compound dependent)
• Electron-impact ionization

Primary use: Measure OCR and ECAR of live cells in a 96-well plate format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells and ex vivo samples

Capabilities

• Four-port injection system and automated mixing allows real-time detection of responses to substrates, inhibitors and other compounds. Temperature is maintained at 37^oC
• A 96-well plate allows multiple conditions to be run at once. The system is optimized for compound screening and dose-response studies.
• As few as 5,000 cells per well may be analyzed at high sensitivity (depending on cell type)
• System is compatible with many different sample types

Specifications

• 96 assay wells
• 2µL microchamber volume